DOI: http://dx.doi.org/10.18257/raccefyn.134

Artículo original

Evaluación de la comunidad desnitrificante nosZ en la columna de agua de Isla del Sol, embalse de Prado, Tolima

Maribeb Castro-González

Resumen


En este trabajo se evaluó la diversidad genética de las comunidades desnitrificantes relacionadas con el reciclaje del gas invernadero óxido nitroso (N2 O) en la columna de agua de Isla del Sol, en el embalse de Prado, mediante el análisis del polimorfismo de los fragmentos de restricción terminal del gen funcional nosZ, que codifica para la reducción de N2 O a N2  durante la desnitrificación. Este es el primer reporte que demuestra la presencia de microorganismos desnitrificantes tipo nosZ en un ecosistema acuático colombiano. Los resultados indicaron que la digestión con la enzima MspI generó un mejor perfil de la comunidad nosZ (expresado en una mayor riqueza de fragmentos de restricción terminal, FRT), que el obtenido con la enzima HhaI. Además, la comunidad de tipo nosZ presentó mayor riqueza y diversidad (índice de Shannon – H′=0,9) de FRT en la profundidad (9m) a la cual se encontraron condiciones subóxicas (1,1 mg/l de O2 ) y altos niveles de nitrato (0,78 mg/l), lo cual podría favorecer la reducción de N2 O a N2. Estos resultados son la base para el desarrollo de investigaciones futuras que permitan establecer la funcionalidad y significancia de estas comunidades en la regulación de las emisiones de N2 O desde esta hidroeléctrica. © 2014. Acad. Colomb. Cienc. Ex. Fis. Nat. Todos los derechos reservados.

Palabras clave


desnitrificación, embalse, gen funcional, suboxia, hipolimnion.

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Referencias


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